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. 2008 Dec 16;6(12):e319. doi: 10.1371/journal.pbio.0060319

Figure 1. Catalase or Superoxide Dismutase Prevented E. coli mazEF-Mediated Cell Death Induced by Inhibitors of Transcription and/or Translation.

Figure 1

E. coli MC4100relA + (WT), MC4100relA +/pQEkatE (WT+KatE), MC4100relA +/pQEsodA (WT+SodA) or MC4100relA +ΔmazEF (ΔmazEF) were grown aerobically to mid-log phase. Cells were incubated without shaking at 37 °C for 10 min, followed by the addition of: (A) rifampicin (20 μg/ml) for 10 min; (B) spectinomycin (1 mg/ml) for 1 h; (C) chloramphenicol (50 μg/ml) for 20 min; (D) trimethoprim (2 μg/ml) for 1h; and (E) nalidixic acid (1 mg/ml) for 10 min. The wild type strain (WT) is represented by black bars and the derivative ΔmazEF strain is represented by white bars. Error bars indicate standard deviation.