Figure 4. Completely Anaerobic Growth Conditions Prevented mazEF-Mediated Cell Death When mazEF Was Activated by Inhibiting Transcription and/or Translation but Not When Activated by DNA Damage.

E. coli MC4100relA ⁺ (WT) and MC4100relA ⁺ΔmazEF (ΔmazEF) were grown under either aerobic or anaerobic conditions. Cells were incubated either aerobically, without shaking, or in an anaerobic jar with: (A) rifampicin (10, 20, or 30 μg/ml) for 10 min or (B) nalidixic acid (2–3 mg/ml) for 10 min.