Table 3. Loci used for MLST and single locus typing.
| Locus | Product | Primer sequence (5′–3′) | Amplicon size (bp) | MLST fragment size (bp) | G+C % | No. of alleles | dN/dS ratio | % VI | π |
| clpA | Clp protease subunit A | clpAF1240: GATAGATTTCTTCCAGACAAAG | 864 | 579 | 25.7 | 9 | 0.308 | 2.94 | 0.01122 |
| clpAr2104: CAAAAAAAACATCAAATTTTVTATCTC | |||||||||
| clpX | Clp protease subunit X | clpXF403: AATGTGCCATTTGCAATAGC | 721 | 624 | 30.7 | 8 | 0.026 | 1.92 | 0.00529 |
| clpXr1124: TTAAGAAGACCCTCTAAAATAG | |||||||||
| nifS | aminotransferase | nifSF1: ATGGATTTCAAACAAATAAAAAG | 696 | 564 | 27.1 | 4 | 0.079 | 1.06 | 0.00446 |
| nifSr719: GTTGGAGCAAGCATTTTATG | |||||||||
| pepX | dipeptidyl aminopeptidase | pepXF449: TTATTCCAAACCTTGCAATCC | 723 | 570 | 28.0 | 7 | 0.15 | 2.45 | 0.0080 |
| pepXr1172: GTTCCAATGTCAATAGTTTC | |||||||||
| pyrG | CTP synthase | pyrGF448: GATATGGAAAATATTTTATTTATTG | 742 | 603 | 31.2 | 7 | 0 | 1.49 | 0.00549 |
| pyrGr1190: CAAACATTACGAGCAAATTC | |||||||||
| recG2 | DNA recombinase | recGF917: CTTTAATTGAAGCTGGATATC | 777 | 651 | 30.6 | 5 | 0.06 | 1.38 | 0.00530 |
| recGr1694: GAAAGTCCAAAACGCTCAG | |||||||||
| rplB | 50S ribosomal protein L2 | rplBF2: TGGGTATTAAGACTTATAAGC | 758 | 624 | 36.1 | 9 | 0.085 | 2.40 | 0.00757 |
| rplBr760: GCTGTCCCCAAGGAGACA | |||||||||
| ospA | outer surface protein A | Fw1: GACACTGCCTCTGGTGATAGC | 302 | 288 | 37.4 | 10 | 0.69 | 14.9 | 0.03050 |
| Rv1: CTTTCCCTTTTCCTTCTTTTG | |||||||||
| Fw2: AAACAAAGACGGCAAATACGA | |||||||||
| Rv2: ATTCAAGCTTGGTGCCATTT | |||||||||
| ospC | outer surface protein C | Fw1: TGAAAAAGAATACATTAAGTGCAA | 470 | 376–385 | 35.7 | 14 | 2.24 | 36.6 | 0.14230 |
| Rv1: TTTTTGGAGTTTCTGCYACA | |||||||||
| Fw2: TGGCTGTGAAAGAAGTTGAGG | |||||||||
| Rv2: GCCACAACAGGACTTGTAAGC | |||||||||
| IGS 5S–23S | N/A | Reference 21 | 225 | 151–183 | 18.6 | 12 | N/A | 9.23 | 0.01806 |
N/A: not applicable.