Skip to main content
. 2008 Nov 5;36(22):7043–7058. doi: 10.1093/nar/gkn796

Figure 5.

Figure 5.

IN activity and oligomerization on short ODN mimicking the viral ends. 1 pmole of associated (12.5 µM initial concentration) and dissociated (0.125 µM) INZn were analyzed for 3′ processing (A) and strand transfer activities (B). Reactions were performed under standard conditions (7.5 mM MnCl2, 20 mM HEPES pH 7.6) for 0 to 120 min. The final NaCl concentration was adjusted to 30 mM in all reaction solutions. IP, integration product; P, processing product. 3′ processing activity detected after 120 min of reaction with both enzyme preparations was analyzed using either MgCl2 or MnCl2 (7.5 mM) and the percentage of activity recovered was reported in C. Results are the mean ± SD (error bars) of three independent experiments.