Table 1.

Summary of the association and activity analyses of the different enzyme preparations

The IN_Zn, IN_LC (purified in presence of 7 mM CHAPS in addition or not of 50 μM ZnSO₄) were compared for self association, 3′ processing, strand transfer, HSI and FSI products after pre-incubation or not (No) with specific (Spe) or nonspecific (NSpe) 21 bp ODN. The presence of oligomers was evaluated by DSS crosslink and western blotting, monomers (Mo), dimers (Di) and tetramers (Te) are reported. In vitro activities were quantified using Image J software or counting of FSI integrant clones. More than 20% of 3′ processing and strand transfer (+++), 5–20% (+), 15–25% of HSI (+++), 5–15% (++), no activity (−), Above 100 FSI integrant clones (+++), 50–100 (++), 10–50 (+). All the activities reported here were obtained under standard conditions (7.5 MnCl₂ for 3′ processing and strand transfer; 7.5 MgCl₂ for concerted integration). Pre-incubations with ODN were performed in presence of MgCl₂ during 16 h.