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. 2008 Nov 5;36(22):7009–7018. doi: 10.1093/nar/gkn843

Figure 2.

Figure 2.

HPLC, MS and UV analyses of the SeG-DNAs. (A) RP-HPLC analysis of 5′-d(GAATCA-SeG-GTGTC)-3′ [monitored at 260 nm (blue) and 360 nm (red)]. The sample was analyzed on a Welchrom XB-C18 column (4.6 × 250 mm, 5 μ) at a flow of 1.0 ml/min and with a linear gradient of 5 to 50% B in 10 min, with a retention time of 7.6 min. Buffer A: 10mM TEAAc (pH 7.1); B: 60% acetonitrile in 10 mM TEAAc (pH 7.1). (B) MS analysis of 5′-d(GT-SeG-TACAC)-3′.Molecular formula: C78H99N30O45P7Se; [M+H]+: 2473.8 (calcd: 2473.6). (C)UV spectra of the SeG-DNAs containing one SeG (ATG-SeG-TGCAC, black), two SeGs (ATG-SeG-T-SeG-CAC, red), and three SeGs (AT-SeG-SeG-T-SeG-CAC, pink).