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Validation of selected pADPr-associated proteins identified by LC-MS/MS using western blot analysis of pADPr immunoprecipitates. The specificity of the pADPr immunoprecipitation using anti-pADPr 10H monoclonal antibodies was evaluated by immunoblot analysis as described in Materials and methods section. The same proteins were not precipitated by normal mouse IgGs, confirming the specificity of the pull-down.
