Table 2.

Melting temperatures (T_m) for TAR-aptamer complexes

Hairpins	Tm (°C)
	Complexed with TAR	Complexed with TARC5	RNA alone
R06	31.3 ± 0.3	24.4 ± 0.4	71.5 ± 0.6
R06G5*a	20.5 ± 0.3 (−10.8°C)	22.0 ± 0.2	69.5 ± 0.6
R06C8*	33.2 ± 0.6 (+1.9°C)	25.5 ± 0.2	71.3 ± 1.5
R06A12*	30.3 ± 0.5 (−1.0°C)	23.9 ± 0.2	71.2 ± 1.3
TAR	–	–	85.3 ± 0.3
TARC5	–	–	83.4 ± 0.5

^aSubscripts refer to 2′-O-methyl residues at the indicated positions. The experiments were performed at 1 µM of each RNA, in a 10 mM sodium phosphate buffer, pH 7.2 at 20°C, containing 140 mM potassium chloride, 20 mM sodium chloride and 0.3 mM magnesium chloride.