Table 3.

Equilibrium and rate constants for TAR-aptamer complexes

Complexes	kon (104 M−1 s−1)	koff (10−3 s−1)	Kd (nM)
TAR–R06	9.2 ± 0.6	0.29 ± 0.11	3.1 ± 0.1
TAR–R06G5*a	7.9 ± 1.5	3.30 ± 0.01	44.9 ± 7.4
TARC5–R06	11.0 ± 0.1	1.48 ± 0.02	13.3 ± 0.7
TARC5–R06G5*	5.8 ± 0.1	2.19 ± 0.01	38.5 ± 1.7

^aSubscripts refer to 2′-O-methyl residues at the indicated positions. TAR and TAR_C5 hairpins were immobilized onto streptavidin-coated sensorchips. The kinetic experiments were performed in the buffer used for the thermal denaturation experiments except that magnesium ions concentration was increased from 0.3 mM to 3 mM and 0.005% P20 surfactant was added. The thermodynamic parameters were determined from direct curve fitting of the sensorgrams as described in Materials and Methods section.