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A hairpin-linked TM:BM oligonucleotide. The 5-methylcytosines are present at each of 10 CpG sites on both top, TM, and bottom, BM, strands of TM:BM, and are indicated by ‘Me’. Each hairpin linker contains a batchstamp common to all molecules processed in a given experiment, and a randomly generated barcode (19). Hairpin-linked molecules have primer binding sites on the top and bottom strands of the oligonucleotide, permitting subsequent PCR amplification.
