Table 4.

Error frequencies for single-stranded molecules under LowMT bisulfite treatment

Protocol	Seq.	% Failed conversions; Counts (95% credible interval)	% Inappropriate conversions; Counts (95% credible interval)
LowMT 4 h	21	37.3	0.31
		149 (32.7, 42.2)	1 (0, 2.5)
LowMT 8 h	52	36.5	1.2
		361 (33.5, 39.6)	7 (0, 2.7)
LowMT 20 h	58	6.5	3.1
		72 (5.1, 8.0)	19 (1.7, 4.8)

Results for each single-stranded molecule were tallied from 10 methylated, CpG cytosines and 19 unmethylated, non-CpG cytosines. The point estimate for each error frequency is given in the first line of each box; the second line shows the raw event count, and the 95% credible interval on the frequency estimate, calculated using the β-distribution. Failed-conversion frequencies and their credible intervals were calculated directly. Inappropriate-conversion frequencies were calculated as the observed percent minus the bisulfite-independent error frequency.