Table 5.

Error frequencies for hairpin-linked molecules under HighMT bisulfite treatment

Protocol	Seq.	% Failed conversions; Counts (95% credible interval)	% Inappropriate conversions; Counts (95% credible interval)
HighMT 40 min	41	8.4	1.4
		187 (7.3, 9.6)	13 (0.13, 2.7)
HighMT 60 min	12	3.2	0.67
		21 (2.0, 4.7)	2 (0, 2.4)
HighMT 90 min	41	0.86	1.7
		19 (0.51, 1.3)	15 (0.29, 3)

Results for each double-stranded molecule were tallied from 20 methylated, CpG cytosines and 54 unmethylated, non-CpG cytosines. The point estimate for each error frequency is given in the first line of each box; the second line shows the event count, and the 95% CI on this frequency estimate. Failed-conversion frequencies and their CI were calculated directly. Inappropriate-conversion frequencies were calculated as the observed percent minus the bisulfite-independent error frequency, as described in Methods section.