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. 2008 Nov 6;36(22):7088–7099. doi: 10.1093/nar/gkn871

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Extraordinary stability of molecular sub-populations of the hairpin ribozyme. Following EMSA separation (a) of fully in vitro transcribed ribozyme into two bands, the T and B species were further separated by D-PAGE (b) into their composite RzA and RzB strands. The four samples T-RzA, T-RzB, B-RzA and B-RzB were then annealed in all four possible combinations to refold the ribozyme, and analyzed by EMSA (c). An image of the second EMSA gel is shown with the fraction of T and B species for each lane indicated to the right of the respective band.