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Time courses of recombination by wild-type (WT) and activated mutant (NM) resolvase. Recombination of pMA21 (res × res) by WT resolvase and of pAL225 (site I × site I) by NM resolvase was assayed under standard conditions. Aliquots were stopped at the indicated times. Products were analysed by electrophoresis after PstI + HindIII digestion (1.2% gel; upper panels), or nicking with DNase I (0.7% gel; lower panels). Annotation is as in Figure 3.
