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. 2008 Nov 10;36(22):7181–7191. doi: 10.1093/nar/gkn885

Figure 5.

Figure 5.

pH dependence of recombination by wild-type (WT) and activated mutant (NM) resolvase. Recombination of pMA21 (res × res) by WT resolvase and pAL225 (site I × site I) by NM resolvase was assayed in buffers with different pH, as indicated (lanes marked B are controls, not treated with resolvase). Reaction products were analysed by agarose gel electrophoresis after digestion with PstI and HindIII. Annotation is as in Figure 3.