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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Biotechnol. 2008 Jun;26(6):650–651. doi: 10.1038/nbt0608-650

Figure 1. Targeted mutagenesis of zebrafish genes with zinc-finger nucleases (ZFNs).

Figure 1

Two ZFNs that target the locus of interest are generated through rational design and screening. mRNAs encoding the ZFNs are injected into zebrafish embryos at the one-cell stage. After cleavage of the target sequence, the endogenous repair machinery can religate the DNA through nonhomologous end joining, which often adds or deletes nucleotides at the cleavage site.