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. 2008 Dec 24;3(12):e4026. doi: 10.1371/journal.pone.0004026

Figure 3. T-cell activation up-regulates cellular levels of HSP60.

Figure 3

A. LNC were stimulated with Con A for 24, 48 or 72 hr, subjected to a 30 minutes 42°C heat shock (HS) or kept at 37°C (None). Cell lysates were prepared and HSP60 expression was analyzed by western blot with specific antibodies, and quantified (in arbitrary units). B. A2b T-cells were stimulated with various concentrations of the target peptide Mt176-90, a control peptide (Mt3) for 72 hr, or with medium alone (None). Cell lysates were prepared and HSP60 expression was analyzed by western blot with specific antibodies, and quantified (in arbitrary units). Two independent experiments produced similar results.