Figure 7. HSP60-specific Anti-ergotypic T-cells control arthritogenic T-cells in vitro.
LNC from Mt immunized rats (2.5×105 per well) were activated with Mt176-90 for 72 hr in the presence of Anti-p277 or Anti-MBP T-cells (5×104 per well). The secretion of IFNγ was determined by ELISA, the results are presented as pg/ml±SEM of triplicate cultures. The differences between the groups were significant (p<0.05) for antigen concentrations higher than 0.1 µg/ml. Three independent experiments produced similar results.