Figure 8. Anti-ergotypic HSP60-specific T cells become anergic after interacting with activated T cells.

Anti-ergotypic Anti-p277 T cells were stimulated for 3 days with irradiated, activated A2b cells (A2b) or with irradiated APC fed with the p277 peptide (APC). The Anti-p277 T cells were maintained for 4 additional days in culture, and stimulated with APC and p277 peptide, Con A or immobilized anti-TCR (αTCR) antibodies. T-cell proliferation (A) and IFNγ (B) release were measured after 3 days. The proliferative responses are presented as the ΔCPM (±SEM) (A), and the IFNγ as pg/ml±SEM (B) of triplicate cultures. Three to five independent experiments produced similar results.