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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: J Periodontol. 2001 Jun;72(6):815–823. doi: 10.1902/jop.2001.72.6.815

Figure 2.

Figure 2

Effective, prolonged gene transfer of Ad2/GFP to cementoblasts. Subconfluent cementoblasts were transduced with Ad2/GFP at an MOI = 100. After 24 hours the cells were subsequently visualized under phase contrast (left panel; A) and fluorescence microscopy (right panel; A). The cementoblasts were then subjected to FACS analysis to determine the percentage of cells transduced by the adenovirus encoding the jellyfish protein green fluorescent protein (GFP) after 1 and 7 days (B). Note the stability of protein expression in the cells at 1 and 7 days after gene transfer with >90% of the cells exhibiting fluorescence intensity above threshold (middle and right panels, respectively; B). Cells that were not transduced by Ad2/GFP fail to fluoresce (left panel; B) The B designation gives the total cell count on the cell sorter, while the C designation gives the number of cells which surpassed the fluorescence threshold (i.e., cells producing GFP).