Fig. 8.

Dynamic interfacial behavior of apoB19 at the EPC/triolein/water interface. A 10 μl triolein drop was rapidly formed into buffer (arrow a), and was coated with a phospholipid (PL) monolayer by addition of Intralipid (arrow b). Excess unbound PL was washed out of the cuvette by continuous buffer exchange (arrow c). Then 15 μg/ml apoB19 was added to the cuvette (arrow d) and the further decrease in interfacial tension was monitored. The elasticity of the interface at each stage was determined by sinusoidal oscillation of the drop volume (denoted by the rapid vertical displacements) as described under EXPERIMENTAL PROCEDURES. Analyses of apoB20.1 under the same conditions were also performed (36); data not shown.