TABLE 1.
Spectroscopic and thermodynamic properties of apoB19 and apoB20.1
| [Θ]222 | Tm | ΔHD | ΔS | λmax | A305/A270 | |
|---|---|---|---|---|---|---|
| deg-cm2-dmol−1 | °C | kcal/mol | cal/mol°K | nm | ||
| ApoB19 | 6,577 | 57.8 | 96.1 | 290.2 | 327.5 ± 1.0 | 1.26 ± 0.04 |
| ApoB20.1 | 6,243 | 56.4 | 71.9 | 218.2 | 328.0 ± 2.0a | 1.30 ± 0.04a |
[Θ]222, mean residue ellipticity at 222 nm; Tm, thermal denaturation midpoint; ΔHD, enthalpy of denaturation; ΔS, entropy of folding; λmax, wavelength of maximum fluorescence emission; A305/A270, tryptophan emission anisotropy ratio.
a
Means ± SE, n = 3, P = NS by Student's t-test.