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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Exp Hematol. 2008 Oct 29;36(12):1604–1615. doi: 10.1016/j.exphem.2008.08.005

Fig. 8. QPCR analysis shows alterations in the expression levels for key regulatory genes in gata1:Δbr-gfp progenitors.

Fig. 8

Gata1 (GFP+) progenitors were isolated from wild-type (gata1:gfp) adult WKM, and a corresponding population of Gata1+ progenitors was isolated from gata1:Δbr-gfp adult WKM by FACS, with DAPI-based exclusion of dead cells. Total RNA was isolated from these cells and cDNA synthesized. QPCR was performed for analyzing expression levels of RNA from c/ebpα, pu.1 and gata1. The data was processed using the 2-ΔΔCT method [26]. The median of each sample was normalized to wild-type (gata1:gfp) and the median average was graphed as fold change in RNA expression. Triplicates were performed in each of 4 independent experiments. P values were determined by T-test, * P<0.001, ** P<0.02.