Skip to main content
. 2008 Dec 19;283(51):35954–35965. doi: 10.1074/jbc.M804717200

FIGURE 3.

FIGURE 3.

Rac1 plasma membrane localization requires prenylation and an intact PBR. mYFP-Rac1 fusions were expressed in COS1 cells and imaged microscopically for YFP fluorescence. A, mYFP-Rac1(WT); B, mYFP-Rac1(K186E), defective for effector (PIP5Kα) binding; C, mYFP-Rac1(C189S), lacking prenylation; D, Lyn-mYFP-Rac1(C189S), myristoylated derivative; E, mYFP-Rac1(6Q), defective PBR; F, Lyn-mYFP-Rac1(6Q)(C189S); G, mYFP; H, Lyn-mYFP, myristoylated mYFP. Note that Lyn myristoylation targets proteins localized nuclearly (C) or perinuclearly (E) to the plasma membrane (F and H, respectively). White scale bar in H, 20 μm (applies to all panels).