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Cross-linking efficiency of CRFR1 mutants. The different mutants were expressed in COS-7 cells; binding and cross-linking to 125I-YQB-SVG was performed as in Fig. 1B. Similar amounts of bound receptor-ligand complex (1200 cpm) were analyzed on SDS-PAGE followed by autoradiography using phosphorimaging (A). In B, cross-linking efficiency was calculated as the ratios of the receptor band of the different receptor mutants divided by the receptor band in the wild type. WT, wild type.
