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. 2008 Dec 19;283(51):35402–35409. doi: 10.1074/jbc.M807045200

FIGURE 2.

FIGURE 2.

Time-dependent inhibition of HDAC1 and HDAC3 as a function of inhibitor 106 concentration. The reaction mixture contained 100 ng of enzyme in the standard HDAC Tris buffer (pH 8.0), 50 μm acetylated-lysine substrate, various concentrations of inhibitor 106 (indicated) and Lys-C peptidase (2 milliunits). A, the progress curves for HDAC1 in the presence of increasing concentrations of inhibitor 106 were measured for 1 h and fit with Equation 1 (left). The rate constant kobs as a function of inhibitor 106 concentration was plotted (right). B, the progress curves for HDAC3 in the presence of increasing concentrations of inhibitor 106 were measured for 1 h and fit with Equation 1 (left). The rate constant kobs as a function of inhibitor 106 concentration was plotted (right).