FIGURE 6.

Characterization of myocardin domains that bind TDG. A, schematic representation of myocardin indicating the GST fusion proteins analyzed. NTD, N-terminal domain; ++, basic domain; Q, poly(Q) domain; LZ, leucine zipper domain; TAD, transcriptional activation domain; ND, not determined; mut, mutant. B, bacterial expressed TDG was incubated with the series of GST-myocardin fusion proteins indicated in panel A. Western blotting was performed to detect the GST-myocardin bound TDG (upper panel). The lower panel in B indicates the expression of the GST fusion proteins as detected by a Ponceau S staining. TDG was found to bind to myocardin NTD (N-terminal domain) and basic (++), poly(Q) domain as summarized in panel A.