TABLE 3.

IC₅₀ values of wild type versus Δpfpnp using various immucillins

Parasite lines were incubated with drug in media without hypoxanthine for 48 h and then further incubated with radiolabel for 24 h. For each line, 3-12 independent experiments were carried out. The error is reported as mean ± S.E. To determine the statistical significance between the IC₅₀ values of the wild type and disrupted lines for a particular inhibitor the Bonferroni test was used to calculate the p value for every combination of group pairs.

Specific activity
Parasite line	IC50 (nm)
	ImmH	MT ImmH	2'-Deoxy ImmH	2'-Deoxy ImmG
3D7	43 ± 5	86 ± 13	440 ± 43	530 ± 41
hrpII integrant	48 ± 8	62 ± 10	480 ± 37	560 ± 16
Δpfpnp 1	8.5 ± 3.7a	840 ± 62a	16 ± 7b	6.4 ± 0.8a
Δpfpnp 2	8.5 ± 3.5a	900 ± 90a	6.0 ± 2.8b	5.9 ± 0.6a

^ap = <0.0001.

^bp = <0.001.