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Comparison of kinetic constants for hCBR1- and hFDH-catalyzed GSNO reduction using NADPH and NADH, respectively
| Enzyme | Substrate | Km | kcat | kcat/Km |
|---|---|---|---|---|
| μm | min–1 | mm–1 min–1 | ||
| hCBR1 | GSNO | 30.1 ± 3.6 | 450 ± 85 | 14,950 |
| hCBR1 | Menadione (4)a | 22 | 402 | 18,272 |
| hFDH | GSNO (19) | 27 ± 8 | 2400 ± 400 | 90,000 |
The standard errors were reported to be <20% for menadione reduction by hCBR1 (19)
