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Quantitation of hydroxylamine, ammonia, and nitrite for reactions prepared with 200 μm GSNO, 200 μm NADH (hFDH), or 200 μm NADPH (hCBR1) in 250 mm sodium phosphate pH 6.8
| NH2OH | NH3 |  |
Unaccounted | |
|---|---|---|---|---|
| μm | μm | μm | ||
| hCBR1 | 30 ± 3 | 4.6 ± 0.4 | <15 | 165 |
| hFDH | 23 ± 5 | 5.7 ± 0.4 | <15 | 171 |
| No enzymea | 14 ± 10 | 1.0 ± 1.0 | 31 ± 1 | 154 |
The background measurements were made in the absence of diethylenetriaminepentaacetic acid
