FIGURE 1.

Intact mass reconstruct of Rpl42ab derived from wild type (BY4742), ΔYbr030w (BY4742), ΔSET7 (BY4742), and the double knock-out ΔYbr030w ΔSET7 strains. Ribosomal large subunit proteins were isolated and separated by reverse phase HPLC as described under “Experimental Procedures.” The resulting effluent was split, with half directed to the source of an electrospray mass spectrometer (PE Sciex III) and the other portion collected in a fraction collector for later analysis. The resulting deconvoluted MS spectra of Rpl42ab are displayed with the average masses of the significant peaks shown.