TABLE 2.
Fourier transform ion cyclotron resonance of intact Rpl42ab
Rpl42ab purified from strain | Observed monoisotopic mass | Calculated monoisotopic mass | Error | Modifications observed | % Abundance of methylation statea | |
---|---|---|---|---|---|---|
Da | Da | Δmass Da | ppm | % | ||
Wild type | 12100.71 | 12100.70 | 0.01 | 0.84 | Two methyl groups | 78 |
12086.70 | 12086.69 | 0.01 | 0.71 | One methyl group | 22 | |
ΔYbr030w | 12086.73 | 12086.69 | 0.04 | 3.57 | One methyl group | 100 |
ΔSET7 | 12086.75 | 12086.69 | 0.07 | 5.38 | One methyl group | 88 |
12072.72 | 12072.67 | 0.05 | 4.34 | None | 12 | |
ΔYbr030w ΔSET7 | 12072.68 | 12072.67 | 0.01 | 0.93 | None | 100 |
Calculated as described under “Experimental Procedures”