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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Oncogene. 2008 Jun 30;27(47):6120–6130. doi: 10.1038/onc.2008.207

Figure 3. Effects of HER2 overexpression on Aldefluor-positivity and tumorigenicity of human mammary carcinoma cell lines.

Figure 3

A. Overexpression of HER2 increased the Aldefluor-positive populations three- to five-fold compared to control DsRed cells in indicated breast cancer cell lines. Knockdown of HER2 in HCC1954 cells using HER2 siRNA decreased the Aldefluor-positive cell population. B. Aldefluor-positive but not Aldefluor-negative cells display properties of tumor stem cells despite equivalent HER2 expression in both populations as shown by Western blotting. Tumors generated from the Aldefluor-positive population are composed of both Aldefluor-positive and -negative populations recapitulating the initial tumor phenotype. C. Growth curves of serial dilutions of Aldefluor-positive and -negative SUM159-HER2 cells. Aldefluor-positive cells form tumors at all cell concentrations whereas the Aldefluor-negative cells failed to do so. D. In HER2 amplified cell lines MDA-MB453, HCC1954 and JIMT-1, only Aldefluor-positive cells were tumorigenic.