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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Microbiol. 2008 Aug 27;70(2):462–478. doi: 10.1111/j.1365-2958.2008.06424.x

Figure 2. Expression of MtrE by wild type and mtr mutant bacteria.

Figure 2

Outer membrane proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane. The 53-kDa MtrE protein was detected by a rabbit MtrE-specific polyclonal antibody described in the Materials and Methods. With the exception of mtrE mutant DW3, protein samples were loaded in ascending order of the levels of antibiotic resistance. The 32-kDa porin protein is constitutively expressed in N. gonorrhoeae and was detected by staining the PVDF membrane with amido black to show equal loading of the samples. The intensity of the MtrE band relative to strain FA19 and after normalization to porin is reported for each strain below the blot. Strain DW11, not described here, carries a frame-shift mutation in the mtrR gene, that is predicted to cause a 52 kD truncated MtrR protein (Warner et al., 2007).