Table 1. Bacterial strains used in this study.
Strain1 | Relevant Genotype | Reference |
---|---|---|
FA19SmR | Parent strain | (Jerse et al., 2003) |
KH15 | -T at MtrR binding site (mtr-79) | (Hagman et al., 1995b) |
DW39 | mtrRA39T (DNA-binding region) | This study |
DW45 | mtrRG45D (DNA-binding region) | This study |
DW9 | mtrRE202G | (Warner et al., 2007) |
JF1 | ΔmtrR | (Folster et al., 2005) |
MS11 | Wild type strain, mtr120, mtrRA39T | (Swanson et al., 1988) |
DW120 | FA19SmR mtr120 | This study |
FA19MS11mtr | FA19SmR mtr120, mtrRA39T | This study |
JF3 | mtrA∷aphA3 | (Rouquette-Loughlin et al., 2002) |
DW120A | FA19SmR mtr120, mtrA∷aphA3 | This study |
JF1 mtr120 | mtr120, ΔmtrR | This study |
DW3 | mtrE∷cat | (Warner et al., 2007) |
DW3MS11 | Strain MS11 with mtrE∷cat | This study |
FA19CmR | cat gene inserted between aspC and lctP loci | (Warner et al., 2007) |
DW39CmR | mtrRA39T and cat gene inserted between aspC and lctP loci | This study |
DW45CmR | mtrRG45D and cat gene inserted between aspC and lctP loci | This study |
DW9CmR | mtrRE202G and cat gene inserted between aspC and lctP loci | This study |
All recombinant strains were constructed strain FA19SmR with the exception of DW3MS11, which was made in strain MS11.