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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5537–5544. doi: 10.4049/jimmunol.181.8.5537

Fig. 1. Binding of C4BP-FITC to clinical isolates and laboratory strains of P. gingivalis.

Fig. 1

Bacteria were grown for 6 days on TSB agar and suspended in the binding buffer at 6×105 cells/ml. A) Wild type strains W83 and W50 were incubated with indicated concentrations of C4BP-FITC for 1h at RT, washed and analyzed by flow cytometry. B) C4BP-FITC (50 µg/ml) was incubated with a number of strains of P. gingivalis for 30 minutes at RT, the cells washed and analyzed by flow cytometry. C) The bacteria were incubated with 5% NHS diluted in GVB++ followed by detection of bound C4BP with mAb 104 and secondary FITC-labelled anti-mouse antibody. Binding to every strain was analyzed three times in duplicates. Shown are mean values +/− standard deviation (SD).