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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5537–5544. doi: 10.4049/jimmunol.181.8.5537

Fig. 4. Binding of C4BP depends on the age of culture.

Fig. 4

Bacteria were grown for up to 8 days on TSB agar, harvested every day starting from day 1 after spreading on plates, washed twice and suspended in binding buffer at 6×105 cells/ml. Afterwards C4BP-FITC (50 µg/ml) was added for 30 minutes at RT, the cells were washed and analyzed by flow cytometry. Binding was investigated at least three times in duplicates for each time point.