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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Immunol. 2008 Oct 15;181(8):5537–5544. doi: 10.4049/jimmunol.181.8.5537

Fig. 5. Bacteria form aggregates during culture, which bind C4BP stronger than dispersed bacterial cells.

Fig. 5

Wild type strain J4261 was harvested on a third and eight day of culture and incubated with 50 µg/ml C4BP-FITC. The binding was detected by flow cytometry. After 8 days of culture a subsets of bacteria forming aggregates appeared in gate R2 (B), which are not to be observed after 3 days of culture (A). These aggregates bound more C4BP (E, F) than single cells after both 3 (C) and 8 (D) days of culture. One representative flow cytometry graph of at least three independently performed is shown for each condition. The bacteria were also visualized using Gram staining after three (G) and eight (H) days of culture.