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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Protein Eng Des Sel. 2008 Jan 31;21(4):223–231. doi: 10.1093/protein/gzm093

Fig. 5.

Fig. 5

Chromatogram for the production of I1 from isolated and unblocked I2 (des-[30–75]) after 1 h. The conditions were: 25°C, 6.6 mM TCEP, pH 3, 6 M GdnHCl, 0.3 mg/ml unblocked I2. The absorbance at 280 nm was monitored. The chromatogram shown in Fig. 1 is superimposed for comparison.