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. Author manuscript; available in PMC: 2008 Dec 15.
Published in final edited form as: Protein Eng Des Sel. 2008 Jan 31;21(4):223–231. doi: 10.1093/protein/gzm093

Table I.

Molecular weights of native ONC and its AEMTS-blocked intermediates, I1, I2 and I3

Observed chromatographic peaks of Fig. 1 Experimental MW of ONC species in Fig. 1, blocked with AEMTSa Theoretical MW of ONC species blocked with AEMTS Number of disulfide bonds in each species in Fig. 1
N 11 820b 11 819b,c 4
I3 11 970 11 971 3
I2 11 971 11 971 3
I1 12 121 12 123 2
R 12 427 12 427 0

Each AEMTS adduct adds 76 Da to the molecular weight of the native protein.

a

Mass measurements were made on a MALDI-TOF MS instrument.

b

N cannot be blocked with AEMTS.