Table I.
Observed chromatographic peaks of Fig. 1 | Experimental MW of ONC species in Fig. 1, blocked with AEMTSa | Theoretical MW of ONC species blocked with AEMTS | Number of disulfide bonds in each species in Fig. 1 |
---|---|---|---|
N | 11 820b | 11 819b,c | 4 |
I3 | 11 970 | 11 971 | 3 |
I2 | 11 971 | 11 971 | 3 |
I1 | 12 121 | 12 123 | 2 |
R | 12 427 | 12 427 | 0 |
Each AEMTS adduct adds 76 Da to the molecular weight of the native protein.
Mass measurements were made on a MALDI-TOF MS instrument.
N cannot be blocked with AEMTS.