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. 2008 Dec 24;3(12):e4020. doi: 10.1371/journal.pone.0004020

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Sasaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Western blot analysis of different Cdks and Cyclins following co-IP with FLAG-SIRT1. Complex formations of FLAG-SIRT1 with Cdk1 and cyclinB were detected, but not with Cdk2, Cdk4, Cdk6, or cyclinA. Amounts of samples used: whole cell extract (WCE), 20 µg; IP 500 µg. B. Western blot of co-IP experiments with endogenous SIRT1, which also forms a complex with Cdk1 and cyclinB. Amounts of samples used: WCE, 20 µg; IP 1.5 mg. WCE, whole cell extracts; IP, immunoprecipitation; FLAG, FLAG-M2 beads.