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. 2008 Dec 24;3(12):e4020. doi: 10.1371/journal.pone.0004020

Figure 8. Phosphorylation of SIRT1 at Thr530 and Ser540 is required for normal cell cycle progression.

Figure 8

A. Fraction of cells in M-phase of the cell cycle in Sirt1+/+ (blue) and Sirt1−/− (red) ES cells at three different time points in culture. The differences were not significant. B. Graphic representation of the fractions of cells in each phase of the cell cycle in Sirt1+/+ and Sirt1−/− ES cells transfected with empty vector (mock), WT-FLAG-SIRT1 (WT), or AA-FLAG-SIRT1 (AA) mutant. C. Table of p-values comparing mock-transfected Sirt1+/+ cells to Sirt1−/− cells transfected with empty vector (Mock), wild-type (WT), or mutant (AA) DNA. *p<0.05; **p<0.01.