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Exercise training decreased LV superoxide generation. LV homogenates were prepared as described in Methods to determine superoxide generation. Activity was assay in a Krebs-HEPES buffer using 5 μM lucigenin, 200 μM NADPH and in the absence or presence of 300 μM apocynin. Values are mean ± SEM cpm/mg protein of 10 animals per group; # p < .05 compared to respective control, * p < .05 compared to respective -BH4 assay group.
