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Viral load in lung tissues of challenged mice was detected by Q-RT-PCR. Viral titers of SARS-CoV in lung tissues from mice i.m. or i.n. vaccinated with a single prime dose (im.P) or prime-boost doses (im.B, in.B) of RBD-rAAV were determined. Mice i.m. and i.n. vaccinated with blank AAV were used as negative controls. The data are expressed as mean + SE of RNA copies per microgram of lung tissue from four mice for each group.
