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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Eur J Neurosci. 2008 Nov;28(9):1867–1876. doi: 10.1111/j.1460-9568.2008.06472.x

Figure 7.

Figure 7

Effect of treatments on LPS-induced expression of TNF-α mRNA and protein in the spleen (A, C) and liver (B, D) as determined by real-time RT-PCR. Re-exposure to the previously heroin-paired environment (CS, conditioned stimulus) reduced TNF-α mRNA and protein in the spleen and TNF-a protein in liver tissue and this reduction was attenuated by inactivation of the BLA. The RT-PCR data (A, B) are expressed as TNF-α copy number per 10 ng cDNA based on a standard curve using Roche LightCycler software. The ELISA data (C, D) are expressed as picograms of protein per ml. (*) Asterick indicates a significant difference (p<0.05) from the HC (Home cage), Saline-BLA group.