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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Virology. 2008 Jul 2;378(2):282–291. doi: 10.1016/j.virol.2008.05.023

Figure 8. Electron microscopy of HN(ecto) and HN(NA) proteins.

Figure 8

Protein samples were negatively stained and visualized as described previously (Connolly et al., 2006). (A–D) Representative images of different projection views of the HN(ecto) protein are shown to the right of a schematic diagram. The images demonstrate variability in both the interdomain packing within the globular head and in the extension of the stalk regions. In these images, the HN globular head exhibits apparently loose and variable dimer of dimer associations, with panels A and B representing “side” views of the HN ectodomain including the stalk and panels C and D representing “top” views of tetramers of the HN NA domains. The stalk regions visible in panels A and B are exhibit variable orientations relative to the NA domains and internal bending consistent with flexibility within this domain. Representative EM images of the HN(NA) protein is shown in panel (E), which appears as single globular and individual dots in the micrographs, in contrast to the florets observed for HN(ecto).