Table 1.
Characterization of the size of PEI-pDNA nanoparticle complexes.
| Group | Amount of DNA (μg/mouse) | PEI Structure | N:P ratio | Size (nm) |
|---|---|---|---|---|
| 1 | 1 | Branched | 5 | 77.9 ± 11.5 |
| 2 | 5 | Branched | 5 | 109.1 ± 2.20 |
| 3 | 10 | Branched | 5 | 136.4 ± 2.84 |
| 4 | 25 | Branched | 5 | 155.2 ± 2.66 |
| 5 | 50 | Branched | 5 | 165.3 ± 4.30 |
| 6 | 100 | Branched | 5 | 238.5 ± 5.26 |
| 7 | 1 | Linear | 5 | 106.3 ± 2.49 |
| 8 | 5 | Linear | 5 | 112.3 ± 8.11 |
| 9 | 10 | Linear | 5 | 122.8 ± 0.85 |
| 10 | 25 | Linear | 5 | 138.3 ± 3.90 |
| 11 | 50 | Linear | 5 | 161.9 ± 3.88 |
| 12 | 100 | Linear | 5 | 265.7 ± 6.40 |
| 13 | 25 | Branched | 1 | 96.2 ± 2.09 |
| 14 | 25 | Branched | 2 | 112.5 ± 1.45 |
| 15 | 25 | Branched | 3 | 128.1 ± 5.35 |
| 16 | 25 | Branched | 10 | 131.1 ± 1.74 |
| 17 | 25 | Branched | 20 | 97.5 ± 1.03 |
| 18 | 25 | Linear | 1 | 433.9 ± 6.76 |
| 19 | 25 | Linear | 2 | 581.7 ± 9.10 |
| 20 | 25 | Linear | 3 | 415.1 ± 3.26 |
| 21 | 25 | Linear | 10 | 141.5 ± 3.26 |
| 22 | 25 | Linear | 20 | 179.0 ± 3.45 |
*
N/P ratio is defined as the ratio of primary amino group in PEI to phosphate group (in DNA).