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. Author manuscript; available in PMC: 2009 Mar 12.
Published in final edited form as: J Mol Biol. 2008 Jul 3;381(4):1012–1024. doi: 10.1016/j.jmb.2008.06.082

Figure 3. CHIR-AB1-FcY binding stoichiometry.

Figure 3

(a) Equilibrium gel filtration analysis of the CHIR-AB1-FcY complex. The column was equilibrated with and run in a buffer containing 2.5 µM CHIR-AB1. Complexes were prepared by incubating 2.5 µM FcY with varying amounts of CHIR-AB1 at receptor:FcY molar ratios of 1:1, 2:1, 3:1 and 4:1. Similar experiments were conducted using columns equilibrated with 1.5µM, 5µM and 10 µM of CHIR-AB1 (data not shown). (b) Scatchard plot including data from equilibration buffers containing 1.5µM, 2.5µM, 5µM and 10µM CHIR-AB1. The best fit line to the data yields a KD of 840 nM and an x-intercept of 1.83.