Fig. 1.

Strand-specific RT-PCR of barley third leaf RNA for the detection of HvCesA6 antisense transcripts. (A) Schematic representing the primer names, positions, and strands used for strand-specific RT-PCR of HvCesA6 to detect sense and antisense transcripts. (B) RT-PCR was performed with first-strand cDNA templates. For Lanes 1-6, short (s), medium (m), and long (l) A6 sense primers were used to detect antisense transcripts, and either A6-antisense-1 or A6-antisense-2 was used as the primer pair in PCR. For Lanes 7 and 8, A6-antisense-1 was used to detect sense transcript, and A6-sense-(m) was used as the primer pair in PCR. For Lanes 9-14, oligodT was used to detect sense transcripts, by using the three A6-sense primers for PCR. A 1-kb ladder (New England Biolabs) is indicated by (M). The inclusion or exclusion of reverse-trancriptase (RT) is indicated by + or −, respectively. Digital photograph of the ethidium bromide-stained gel is shown in inverted contrast for clarity. Identity of the PCR products was confirmed by DNA sequencing.