Fig. 5.

Blocking coupling factors reduces osteoclast conditioned media-induced hMS cell mineralization. RAW 264.7 precursor (−RL) and multinucleated RAW 264.7-derived osteoclast (+RL) conditioned media were assayed for their effects on osteoblast mineralization. DKK1, control antibody (CONT Ab), αBMP6 antibody, vehicle for the S1P receptor 1 antagonist (VEH), or a S1P receptor 1 antagonist alone or in combination were added to the osteoclast conditioned media as indicated for 7 days before fixing, Alizarin red staining, and quantitation as detailed in Experimental Procedures (n = 3 replicates). *, P < 0.05 compared with vehicle. These results are representative of 2 experiments.